Sorting Hybridoma Cells
Neal Benson (nbenson@nervm.nerdc.ufl.edu)
Fri, 30 Jun 95 17:22:06 edt
One of my lab's users has several hybridoma cell lines that they would like
to re-clone to improve antibody production. It was suggested that they use
cell sorting to select the "high-expressers". They propose to label the
cells with a FITC-labeled anti-mouse IgG (subclass specific), then sort
those in the upper tail of the resulting fluorescence histogram.
In my limited knowledge of this area, I seem to have the impression that
expression of antibody on the cell surface does not necessarily correspond
to the quantity of antibody secreted from a hybridoma cell. I'd be very
interested to obtain opinions as to whether sorting of these cells based on
high surface expression is a reasonable thing to do for improvement of
antibody production.
Thanks in advance,
Neal
=======================================================================
Neal A. Benson Tel: (904) 392-0008
University of Florida Fax: (904) 392-4693
Department of Pathology Email: nbenson@nervm.nerdc.ufl.edu
Box 100275
Gainesville, FL 32610-0275 USA
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
