Staining of paraffin sections
Frederic Preffer (preffer@HELIX.MGH.HARVARD.EDU)
Fri, 30 Jun 1995 16:55:49 -0500 (EST)
>
>On Thu, 29 Jun 1995, Frederic Preffer wrote:
>
>> >
>>
>> Why not just stain for the cytokines? (i know this can be done in the mouse,
>> and i am pretty sure anti-human mabs exist).
>
>My guess is that the levels would be too low for detection; also
>permeabilization would be needed to get the antibodies intracellular, so
>that one could not isolate the cells viably.
>
>Steve Gore
>
While i dont remember if isolation was needed, in the original question,
permeabilization/detection seems quite feasible..see Elson et al. J.Immunol
#154:4294-4301 1995
frederic preffer
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Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Cox5
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Massachusetts General Hospital
Boston MA 02114
(617) 726-7481 {fax x2365}
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