RE: Blood Differential Count
Ric_Thorpe_at_APPLIC1@ccmail.llu.edu
Wed, 07 Jun 95 17:58:35 PST
In reply to Rekha Shah regarding a differential count using flow
cytometry. There is an excellent article by Lauralynn Lebeck in the
1993 Sysmex Journal International, Vol. 3 No 1, p 61-69.
In a nutshell, this looks at CD45, CD13, CD14, FSC and SSC. This
correlates well with the traditional manual dif for neutrophils,
lymphocytes, and eosinophils. Monos are slightly higher, owing
largely to a preferential loss of monos in the traditional wedge
smear. Monos (by flow) correlate well with the "spun blood film prep"
method, which is less hazardous to their health.
A slightly adapted version of this has become our method of choice.
Ric Thorpe
Loma Linda University Immunology Center
Loma Linda, CA
Voice: (909) 824-4144
Fax: (909) 478-4144
>Is there any way to get blood differential count by flow cytometry.
>I am working on getting a literature search done. Meanwhile I
thought of >getting input from experts on this forum. I have seen
lots of great >exchanges here.
>Rekha Shah
>CIIT
>6 Davis Dr.
>Pob 12137
>Research Triangle Park NC 27709
>Voice: (919) 558-1370
>Fax: (919) 558-1300
>CIIT Home Page: http://www.ciit.org
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
