Re: c-myc and flow cytometry
STEVEN DAVID GORE (sdgore@welchlink.welch.jhu.edu)
Mon, 5 Jun 1995 09:11:34 -0400 (EDT)
Chris requested experience with flow for c-myc detection:
Mike Kastan developed an assay following formaldehyde fix and triton
X-100 permeabilization which he published in blood (Blood 74: 1517,
1989). He used a corrected MFI to quantify, noting that different bone
marrow populations have different FITC avidity when permeabilized. Mike's
technique was strengthened by his controls, which involved preincubating
the antibody with the peptide to which it was raised.
Steve Gore
Johns Hopkins Oncology Center
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
