Re: apoptosis-Annexin V or anti-PS mAb?
Ray Hicks (hicks@icrf.icnet.uk)
Thu, 1 Jun 1995 12:05:37 +0100 (BST)
Karel wrote:
My question is: does anybody know if there is a difference between
Annexin V
and monoclonal Ab against phosphatidylserine in labelling early apoptotic
cells? Are these reagents commercially available?
Thank you in advance
Karel Drbal
Yes there are several differences the main one being that annexin V is a
[Ca++] dependent binding peptide with very high affinity, removal of
calcium from the buffer causes annexin V to fall off the cell rapidly
allowing restaining after sorting. (annexin V can be fixed to the cell at
high [Ca++] if you don't want it to fall off yet wish to use a low
[Ca++] buffer after staining. I don't know about the availability of Ab but
annexin V is available conjugated to fluorescein from BRAND Applications
BV, and requests for information and supply can be made by fax +31 4499
2165 (Netherlands)
Ray
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