apoptosis-Annexin V or anti-PS mAb?
Karel Drbal (drbal@biomed.cas.cz)
Wed, 31 May 95 11:25:38 +0200
On Tue, 30 May 1995, Ray Hicks wrote:
>You could try Annexin V, which picks up changes in membrane lipids
>(specifically PS) which correlates well with other methods of apoptosis
>detection. It works on intact unfixed cells and detects rearrangement of
>the lipids before loss of membrane integrity. You should be able to
>double label with annexin V-FITC and cd34-PE with little or no problem.
>Ray
My question is: does anybody know if there is a difference between Annexin V
and monoclonal Ab against phosphatidylserine in labelling early apoptotic
cells? Are these reagents commercially available?
Thank you in advance
Karel Drbal tel.: +42-2-4752589
Institute of Molecular Genetics fax.: +42-2-4727979
Academy of Sciences of the Czech Republic e-mail: drbal@biomed.cas.cz
Videnska 1083
142 20 Praha 4
Czech Republic, Europe
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