Anti FITC Antibody kills fluorescence ???

Matthias Haury (mhaury@pasteur.fr)
Wed, 31 May 1995 19:35:07 +0100

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Bakul I. Dalal MD FRCPC FACP FASCP: "TdTtesting"
Previous message: Matthias Haury: "Intracellular Cytokine Staining Methods ??"

Well it's me again, just on another subject, have been asked if the
available Anti FITC antibodies kill FITC fluorescence after binding to FITC
(I have no idea if or if not) ??? For example if one stains with a FITC
coupled antibody and then comes with Anti FITC coupled to PE, can you see
both fluorescences or is the FITC green gone ??

Thanks you again.. Ciao Matthias

______________________________________________________________

Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry _/ _/ _/ _/ _/
Department of Immunology _/ _/_/_/ _/_/_/
Institut Pasteur _/ _/ _/
25 rue du Dr. Roux _/_/_/ _/ _/
F - 75724 PARIS Cedex 15 INSTITUT PASTEUR PARIS
France
Tel: 33 1 45 68 85 38
Tel: 33 1 40 61 31 29
Fax: 33 1 45 68 86 39
Email: mhaury@pasteur.fr

Next message: Bakul I. Dalal MD FRCPC FACP FASCP: "TdTtesting"
Previous message: Matthias Haury: "Intracellular Cytokine Staining Methods ??"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu