B/W overlays on HP LYSYS

Nicholas King (nickk@med.su.oz.au)
Thu, 25 May 1995 09:08:37 +1000

Simon Monard writes:
Can anyone tell me if there is any software out there that can do
histogram overlays using "dotted" and "dashed" lines for making black and
white lines for publication purposes. All our cytometers have HP 340
computers, I have access to MACS and PCs but very little money.

There is a long-winded way on a MAC: (devised by a fellow poor scientist)
Scan the indivdual histogram output from the HP printer as a PICT file and
open the PICT file into Canvas. These histograms must be scanned in
perfectly straight. Run a trace, change each individual histogram line to
what you wish (dotted,dashed, solid - you can make it to your specs in
Canvas), then exactly overlay all the histograms using the axes as a base
(this is critical for obvious ethical reasons). Depending on the quality of
your original material and your scanner, the axes may scan in poorly so at
the end you may have to construct solid axes using the line drawing tools
exactly over the top of the old ones (one of the reasons that you have to
scan them in straight). Group the histograms and new axes and delete the
old axes. I have probably left out one or two steps but you get the general
picture, I'm sure. You can annotate this in any way you wish.
Caveats: 1. You need to be obsesssssssional about matching the individual
histograms, lest someone accuse you of "doctoring" the data. If you have
done it correctly, an extra (untraced) scan of all the overlaid histograms
from the HP output can be shown to overlay exactly on the CANVAS
'manufactured' overlays. Obviously, you should also put in quantitative
data obtained from the LYSIS program on the HP to back up the visuals.
2. You need a good knowledge of Canvas; sometimes things don't go as
planned, and the less you know the longer it takes (or retakes), but with
care, in the end the picture will print out beautifully on a laser printer.

Most places now have a scanner attached to a MAC and a copy of Canvas.
Hope this may help,
Regards,
Nicholas King

Nicholas King
Department of Pathology
Blackburn Building, D06
University of Sydney
New South Wales 2006
Australia.

nickk@blackburn.med.su.oz.au
ph.61 2 351 4553
FAX: 61 2 351 3429


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