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Stephen P. Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gide Drive
Rockville, MD. 20850
__________________________________________________________________________
Subject: CD45-gating
Author: Marc.Langweiler@dartmouth.edu (Marc Langweiler)
Date: 5/24/95 5:28 PM
Greetings,
This question may be more philosophical than factual, and if it seems that I
should know better, turn your burners to high.
Assume that for immunophenotyping
1) you use CD45 as a third color,
2) you gate on CD45 vs SS, and
3) you report results on the other two markers based on discrete CD45 vs SS
bitmaps.
Regarding the reporting of numerical values for CD45:
1) isn't it nonsensical to report a value for CD45 when it itself is a bitmap
parameter, or,
2) doesn't it stand to reason that if you draw a bitmap around a discrete CD45
population, that by definition that population is 100% "positive", and should
be reported as such?
Thanks for your indulgence. My protagonist in this struggle and I have reached
an impasse. I'm hoping that there is a published convention on how to deal
with this issue.
Marc Langweiler
marcl@dartmouth.edu
http://mmm.dartmouth.edu/pages/marcl/flowcyt.html
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