TdTshortcuts

Bakul I. Dalal MD FRCPC FACP FASCP (dbak@unixg.ubc.ca)
Tue, 23 May 1995 14:48:21 -0700

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Running TdT in leukemia immunophenotyping panel increases the turnaround
time considerably (From 4-5 hours to 9-10 hours). Too many washes etc. We
follow Pietta's protocol (Cytometry 1994), using monoclonal Supertech
antibody (single, not the mix).

Has anyone come up with shortcuts for the TdT staining?

Bakul I. Dalal MD FRCPC FCAP FASCP
Division of Hematopathology, LSP1
Vancouver Hospital and Health Sciences Center,
910 West 10th Avenue,
Vancouver, BC, CANADA

Next message: Michael: "CD34's"
Previous message: N.J.Goulding: "Re: ALERT:NIH SUPPORT"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu