Re: Surface Charge

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Howard Shapiro wrote>
>Many years ago Gunther Valet used fluorescein-tagged polyionic
>materials to estimate surface charge; would a similar approach work for
>you? -Howard
>
This should work; except fluorescein is the wrong dye. As Alan Wagonner,
now at Amersham Life Science in Pittsburgh, has pointed out, increasing the
charge on a dye will inhibit stacking and thus, decrease fluorescence quenching.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu