Re: PerCP
Mario Roederer (Roederer@Darwin.Stanford.EDU)
Fri, 10 May 1996 13:54:16 -0800
Nan Harvey writes:
> With some of the tandem conjugates for FL3, I have to
> compensate 5 to 15% to get them out of FL2 and may see
> spill over into FL1.
But...
Since the spillover of tandems into FL1 is due to unquenched PE emission, as
long as you are doing proper compensation between PE and FITC (FL2 to FL1) as
well as the tandem and PE (FL3 to FL2), then there cannot be any uncorrected
spillover between FL3 and FL1.
mr
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