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I've read this in several places, that one must make fresh PF solutions. I
have used 2% PF in PBS over a year old (stored dark at 4C) and have not
noticed any problems. I have also analyzed samples a month or two after
fixing without problems, and no apparent signal (FITC) degradation. Is
there any hard data that shows that PF solutions go bad? If so, what
happens to it? Is there any reference for this?
--Rich
^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^~^
Richard Moldwin, M.D., Ph.D.
Section of Pediatric Hematology/Oncology
University of Chicago Hospitals
Wyler Room C408, MC 4060
5841 S. Maryland Ave.
Chicago, IL 60637
E-mail: rmoldwin@midway.uchicago.edu
WWW: http://rmoldwin.bsd.uchicago.edu
Phone 312-702-6808
Fax 312-702-9881
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