Paraformaldehyde fixative
Libutti, Daniel A. (dal7@CIDDBD2.EM.CDC.GOV)
Mon, 29 Apr 96 09:17:00 EST
Scott;
First, make it fresh. I make a fresh batch each week and store @ 4C.
For 2% I dissolve 2g paraformaldehyde in 75 mL PBS, add a few drops of 5M KOH
until dissolved, adjust pH to 7.0 with HCl.
Daniel
CDC
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Date: Sat, 27 Apr 1996 13:50:26 -0400
From: "T. Scott Thurmond" <thurmons@ehsct7.envmed.rochester.edu>
Subject: Paraformaldehyde fixative
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To the list,
Does anyone have a favorite recipe for paraformaldehyde as a
fixative for membrane antigens? I have gotten recipes from several
sources which range from the ridiculously complex to the ludicrously
simple (i.e., 1% paraformaldehyde in Dulbeccos's PBS). My question is
what should I take into account when selecting a fixative recipe and
what should I avoid (I will be fixing peritoneal cells for B
lymphocyte markers).
Any input would be appreciated.
Scott Thurmond
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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