Non-specific binding
Peter Chapple (peterc@petermac.unimelb.edu.au)
Tue, 23 Apr 1996 16:19:22 +1000
I'm sure that I am not alone in noticing that some antibody clones have a
higher level of background staining than others. In my case, I have a
particular FITC conjugated CD19 (gamma 1) which appears to have a
substantially higher background labelling than my gamma1 negative control
(approx half a decade higher).
My question is what explaination of this phenomenon do people offer to end
users (most notably Pathologists) who want to know what the fundamental
biology of this interaction is ??.
I have shown with various gating demonstrations that the background level is
constant for different sub-populations of cells (lymphoid, granulocytic,
monocytic and *blast cells*) - so it is apparently unrelated to Fc receptor
presence/density.
A plausible explaination will save me many hours of talking
Thanks in advance.
Peter Chapple
Peter MacCallum Cancer Institute
Melbourne AUSTRALIA
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