re: Membrane polarization

Michael Fox (MFox@vines.ColoState.EDU)
Tue, 23 Apr 96 10:19:06 MDT

Jan Bly inquired about measuring fluorescence polarization using TMA-DPH with
flow cytometry. Tom Delohery and I published a paper on fluorescence
polarization using DPH in Cytometry 8: 20-25, 1987 describing how to set up a
Coulter EPICS instrument and what the filter requirements are. While the BD
machine you have will be somewhat different, the basic principles are the
same. Later studies I have done with Joe Dynlacht using TMA-DPH to measure
membrane fluidity use the same filters and principles. I suggest you read
this paper and then let me know if you have other specific questions.

Mike Fox
Colorado State University

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu