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> I'm trying to help a colleague set up some assays for H-Y antigen
> expression. We want to distinguish male host from female donor lymphocytes
> in human liver allografts.
>
> Am having trouble locating an antibody for anti-human H-Y antigen (our
> Linscott's Directory is four years old).
>
> 1.Can anyone recommend a reliable source for anti-H-Y?
H-Y is a classic "minor histocompatibility antigen." One of the
hallmarks of minor histocompatibility antigens is that they are MHC-re-
stricted. Thus it is very difficult, if not impossible, to generate anti-
body-mediated reponses to them. Pick up the September 15, 1995 issue of
Science or the August 24th, 1995 issue of Nature for two recent publica-
tions regarding H-Y. Actually, this is the the only significant data
regarding H-Y in years...
1. von Boehmer H.
Immunogenetics. Female anti-male attack [news; comment].
Nature, 1995 Aug 24, 376(6542):642.
Pub type: Comment; News.
(UI: 95379943)
2. Scott DM; Ehrmann IE; Ellis PS; Bishop CE; Agulnik AI; Simpson E; Mitchell
MJ.
Identification of a mouse male-specific transplantation antigen, H-Y [see
comments].
Nature, 1995 Aug 24, 376(6542):695-8.
3. Pennisi E.
Long-sought H-Y antigen found [news; comment].
Science, 1995 Sep 15, 269(5230):1515-6.
Pub type: Comment; News.
(UI: 95397143)
4. Wang W; Meadows LR; den Haan JM; Sherman NE; Chen Y; Blokland E; Shabanowitz
J; Agulnik AI; Hendrickson RC; Bishop CE; et al.
Human H-Y: a male-specific histocompatibility antigen derived from the
SMCY protein [see comments].
Science, 1995 Sep 15, 269(5230):1588-90.
> 2.Has anyone looked at H-Y antigen density on various human tissues,
> especially leukocytes?
In light of the fact that antibodies cannot be generated to H-Y,
I don't think that you will find this to be a relevant question for your
study.
> I'll appreciate any help you can offer. Thanks in advance.
>
> -Karen
>
> Oregon Health Sciences University
> Portland, Oregon
> henellk@ohsu.edu
Hope that helped!
-- Unique ID : Ladasky, John Joseph Jr. Title : BA Biochemistry, U.C. Berkeley, 1989 (Ph.D. perhaps 1998???) Location : Stanford University, Dept. of Structural Biology, Fairchild D-105 Keywords : immunology, music, running, Green
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