Re: New "Flo-wer" with some basic questions.
steven micko (steven_micko@email.eushc.ORG)
Thu, 21 Mar 96 16:24:11 EST
1. If I optimize and color compensate my FACScan using FITC, PE,
and PerCP labelled cells, is it possible and/or recommended to
acquire/analyse a sample in a 3-color panel which contains only
two mAb's ??
You can run two compensated colors after setting up for 3-color;
however, you might take a look at what effect there is on FL3
compensated signals when you change the compensation for FL2 -
FL1. Theoretically, there should not be any overlap between FL1
and FL3, but ...
2. Isotypic controls:
a) Can one combine two (or more) differing isotypes
(eg/ IgG1 and IgG2a) conjugated to the same fluochrome
(eg/ FITC) in one tube.
SO, I was wondering if all 5 isotypic controls needed could
be batched together in one tube???
If you put two different isotypes labeled with the same fluorochrome
in the same tube and got some nonspecific binding, then to which
isotype would you attribute the NSB signal?
Steve Micko
Emory Hospital
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