New "Flo-wer" with some basic questions.

dladouceur@ISDTCP3.HWC.CA
Wed, 20 Mar 1996 16:47:26 -0500

Hello fellow flo-wers;
I've been trying to set up a three-color panel to run on
peripheral blood of cynomolgus monkeys...and in the process have come
up with some questions that I'm not sure about:

1. If I optimize and color compensate my FACScan using FITC, PE,
and PerCP labelled cells, is it possible and/or recommended to
acquire/analyse a sample in a 3-color panel which contains only
two mAb's ??

2. Isotypic controls:
a) Can one combine two (or more) differing isotypes
(eg/ IgG1 and IgG2a) conjugated to the same fluochrome
(eg/ FITC) in one tube.

Boy Oh Boy...its not easy to explain electronically...let me give
you more details:
- In my panel I will have mAb's which have isotypes, IgG1
labelled with FITC, PE, and PerCP.
- I also have a mAb w/ an IgG2b isotype, PE labelled
and a mAb w/ an IgG2a isotype, FITC labelled

SO, I was wondering if all 5 isotypic controls needed could
be batched together in one tube???

I've been looking for some information in the litterature about
this matter with no success as of yet. I'd appreciate whatever
guidance or references you may be able to give this new "flo-wer"
concerning the above.

Thanks, Diane
E:Mail = <dladouceur@isdtcp3.hwc.ca>

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