Re: quantifying mitochondria

steven micko (steven_micko@email.eushc.ORG)
Thu, 21 Mar 96 16:24:16 EST

hello all hope your enjoying the first day of spring

Here in Atlanta, it snowed on the first day of spring, and I wore three coats to
catch the bus to work this morning.

i recently had an inquiry from a colleague on the possibility of measuring
(qualitative or relative measures are ok) mitochondria in fetal rat hind
muscle tissue. i suggested that if he could obtain single cells we could
most likely measure the relative mitochondrial content in treated vs.
untreated rats.

The dye Red 613 comes to mind. Does it stain mitochondria
stoichiometrically enough so that you can use it to quantitate
mitochondria?

Steve Micko
Emory University Hospital


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