Re: problem with harvesting cells after sorting

(cawort01@roentgen.bcc.louisville.edu)
Fri, 15 Mar 96 10:55:15 EST

what are you sorting the cells into? if you are using plastic tubes you are getting what I jokingly call "like-Liked". the tube will gradually pick up the charge of the droplets. and when that charge gets high enough your sorted cells will be repelled by the tube.(not a good situation<G>). all you need to do is use glass tubes. I hope this helps.

Chris

Connecting@Warp.SPEED


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu