PI vs. 7-AAD Nuclear Staining
Dan Smith (DJS@ALLP.COM)
Fri, 15 Mar 96 10:24:00 PST
I have been trying to surface stain apopotic MCF-7 breast cancer cells with
Annexin V-FITC and stain the DNA with PI to test for necrosis vs apoptosis.
Even though the emission spectra shouldn't overlap I am having to
compensate for what appears to be overlap in both FL1 and FL3.
Also the PI appears to be passing through the membrane of viable cells.
Any suggestions on either problem (Compensation or viability stain)
Would switching to 7-AAD be a possibility.
Thanks for the input,
Dan Smith
San Diego
djs@allp.com
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