Re: Quantifying LIVE CELLS

Deborah Berglund (umbbd@gemini.oscs.montana.edu)
Fri, 15 Mar 1996 11:46:33 -0700 (MST)

A good viable cell label is Fluorescein diacetate. It is taken up by
live cells, broken down inside the cells by nonspecific esterases, at
which point it becomes fluorescent. I use a stock solution of 1mg/ml and
use 10ul per ml of cells.

Deb Berglund
Montana State University


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