Quantifying LIVE CELLS

Osama Nabulsi (Osama.Nabulsi@amgen.com)
13 Mar 1996 18:19:06 -0800

REGARDING Quantifying LIVE CELLS

I am planning to run a survival bioassay were then I can quantitate LIVE =
cells only using the flowcytometer.
I called Molecular Probes and they suggested "SYTOX" which stains dead =
cells only (but will not cross the membrane of live cells). Have anyone =
used this stain before? Advantages or Disadvantages?
or is there a live cell staining? Suggestions......

Osama Nabulsi
AMGEN INC.
(805)447-1594


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu