Apoptosis with Hoe342 ratio

John Dunne 855-5504 (Jack.Dunne@syntex.com)
11 May 1995 12:14:04 -0800 (PST)

Hi kids!
Jaume asks for help with Hoescht 33342 ratiometric apoptosis
assays. Laura Chiu and I have been working in this area for a
while. I think it's a very powerful and easy method, and
would be glad to discuss technical details. Interested
nozzle-washers should check out:

Watson, J. V, A. Nakeff, S. H. Chambers, and P. Smith. 1985.
Flow cytometric fluorescence emission spectrum analysis of
Hoescht-33342-stained DNA in chicken thymocytes. Cytometry
6:310.

Stokke, T. and H. B. Steen. 1985. Multiple binding mondes for
Hoescht 33258 to DNA. J. Histochem. and Cytochem. 33:333.

Ellwart, J. W, and P. Dormer. 1990. Vitality measurement
using spectrum shift in Hoescht 33342 stained cells. Cytometry
11:239.

Belloc, F. P. Dumain, M.R. Boisseau, C. Jalloustre, J.
Reiffers, P. Bernard and F. Lacombe. 1994. A flow cytometric
method using hoescht 33342 and propidium iodide for
simultaneous cell cycle analysis and apoptosis determination
in unfixed cells. Cytometry. 17:59.

If anybody knows of other relevant references, please post
them.

Happy Pulses,
Jack.Dunne@syntex.com
415 855 5504

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