Green Fluorescent protein
Dean Hewish (deanh@tigger.mel.dbe.CSIRO.au)
Fri, 28 Apr 1995 08:37:17 +1000
>Kirk Watkins writes
>A while back there was some discussion about "Green Fluorescent Protein".
> Someone has asked me about using it for flow analysis of transfected sperm.
>What's the excitation/emission?
The native protein is excited at 396 and 475nm (minor) and has emission at
509nm.
Changing a single amino acid gives rise to an improved form with excitation
at 490 and emission at 510nm (Nature 373 663-664).
I have been trying to get our molecular biologists to try the GFP but they
are a conservative lot and hooked on Glutathione Sulpho-Transferase, which
is a pain to analyse by flow.
Dean R. Hewish, Cell Biologist & Flow Cytometrist.
CSIRO Biomolecular Engineering, 343 Royal Parade, Parkville, 3052,
Victoria Australia.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
