Flow cytometry and lipid peroxidation ?

Brunet Sylvain (brunetsy@ERE.UMontreal.CA)
Wed, 26 Apr 1995 21:00:40 -0400 (EDT)

Greeting to everyone, am i glad to finaly meet people interested by flow
cytometry!

I tought that someone migth be able to help me, so here i am asking your
help!

I've been looking all around to find a flow cytometric technic to
assess lipid peroxidation in cells suspension. Does anyone know of such
technics ? I know that you can load cells with parinaric fatty acid and
then measure the loss of fluorescence along with the peroxydation process,
but i cant use this approach. You see my protocol requires that i measure
an index of peroxidation in ex-vivo cells treated in-vivo with a
pro-oxidant compound. Help me please! Tell me where to look !!!

Thank you.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu