Re: Annexin-FITC Staining Apoptotic Cells

Ray Hicks (hicks@icrf.icnet.uk)
Mon, 24 Apr 1995 15:00:21 +0100 (BST)

On Fri, 21 Apr 1995, Jeff Clapper wrote:

> As recommended by N.J. Goulding, I read with great interest Koopman's 1994
> paper in Blood about surface staining apoptotic cells with annexin-FITC.
>
> I was wondering if anyone has tried this in conjunction with
> fluorescent immunophenotyping as a way of identifying which classes
> of lymphocytes are undergoing apoptosis?
>
> I am thinking of using the annexin-FITC along with various PE and CyChrome
> labelled CD markers. Any comments or suggestions?
>
> >From where can I buy some annexin-FITC already prepared?
>
> Jeff Clapper
>
> Iowa State University
>
>
>
>
Annexin V FITC was developed by Chris Reutelingsperger
<C.Reutelingsperger@BIOCH.RULIMBURG.NL>, it is
being supplied commercially by BRAND Applications BV, and requests for
information and supply can be made by fax +31 4499 2165 (Netherlands)

Ray


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