Annexin-FITC Staining Apoptotic Cells

Jeff Clapper (clap@iastate.edu)
Fri, 21 Apr 1995 11:57:56 CDT

As recommended by N.J. Goulding, I read with great interest Koopman's 1994
paper in Blood about surface staining apoptotic cells with annexin-FITC.

I was wondering if anyone has tried this in conjunction with
fluorescent immunophenotyping as a way of identifying which classes
of lymphocytes are undergoing apoptosis?

I am thinking of using the annexin-FITC along with various PE and CyChrome
labelled CD markers. Any comments or suggestions?

>From where can I buy some annexin-FITC already prepared?

Jeff Clapper

Iowa State University


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