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> I'm looking for protocols and techniques to identify apoptotic cells on the
> FACS. Any methods or references are wellcome (I want to evaluate a bit
> what's already existing before deciding if I have to develop a new one...).
>
********
Hi there,
We have come across a novel method for detecting apoptotic cells. It is
based upon increased phosphatidyl serine exposure on the outer leaflet
of the plasma membrane by cells undergoing apoptosis. This can be
detected using FITC-labelled annexin V.
The method is published by:-
Koopman G. et al Blood 1994 84,1415-1420
This method works nicely for lymphocytes in our hands. With PI
dual staining you can also distinguish apoptotic from dead cells.
All the best
Nick Goulding
Arthritis Research Unit,
St. Bartholomew's Hospital, London
00 44 171 982 6128
Fax 00 44 171 982 6076
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