Re: blocking Fc receptors
Monika (Monika@miltenyibiotec.de)
20 Apr 1995 16:36:29 +0200
Nachricht von RE> blocking Fc receptors
Here comes the help (hope so)!
I'm working in the group of Andreas Radbruch in Cologne together with Mario Assenmacher
and JŸrgen Schmitz. They have developed a method for intracellular staining of
cytokines and have much experience in this business. They have no problems with
high background staining. Here are some references from which you can get
information about their method and the results:
Schmitz, J., Assenmacher, M. and A. Radbruch. 1993. Regulation of Th cell
cytokine expression: functional dichotomy of APCs. Eur. J. Immunol. 23: 191
Assenmacher, M., Schmitz, J. and A. Radbruch. 1994. Flow cytometric determination
of cytokines in activated murine Th lymphocytes. Expression of IL-10 in
IFN-gamma and in IL-4 expressing cells. Eur. J. Immunol. 24: 1097
A. Radbruch. Flow Cytometry and Cell Sorting. Springer Laboratory. ISBN
0-387-55594-3
If you think it's interesting for you and you need more detailed information you
can send me your Fax-No. and I will send you the protocol.
Good Luck ;-)
AlexanderH@MiltenyiBiotec.de
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
