K/L reagents

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• Next message: Brian Smith: "Re: kappa lambda analysis"
• Previous message: Jonni Moore: "RE: kappa lambda analysis"

I'd be appreciative if you could post any responses you get that are not sent
through Purdue.

I have struggled and continue to do so with this issue. I prefer to use a
three tube, three-color analysis:
1) isotyope/19/45
2) kappa/19/45
3) lambda/19/45

I prefer to use monoclonal reagents. I had been using Caltag's PE*conjugated
light chains, but their lambda*PE just went south, so I've switched to using
their FITC light chains. It seems that their lambda is better in FITC and kappa
is better in PE.

I'm really not happy with their lot-to-lot variation, but have yet to find an
_affordable_ alternative for monoclonal reagents,

I have contacted a company called Chromaphobe, who carry these reagents, but
after promising to send me a catalog several weeks ago, they've failed to
follow up.

Thanks for any help you might offer.

Marc Langweiler voice (603) 650-4924
DMS Pathology fax (603) 650-6120
marcl@dartmouth.edu
http://mmm.dartmouth.edu/Pages/marcl/flowcyt.html

• Next message: Brian Smith: "Re: kappa lambda analysis"
• Previous message: Jonni Moore: "RE: kappa lambda analysis"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu