removal of IgG from fc receptors
Antony Bakke (bakkea@ccmail.ohsu.edu)
Mon, 10 Apr 95 09:36:47 PST
Removal of IgG from Fc receptors can be accomplished in one of
two ways: 1) use low pH (3-4) as another respondant replied, or
2) use warm washes. This is less toxic to the cells. Incubate
cells for 30 min at 37 degrees C in media with 10% fetal calf
serum. Then wash 2-3 times by centrifugation with the same
warmed media.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
