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J Phillip asks:
> One of the PI's from my department raised the question of "how to
> remove endogenous IgG from fc receptors?" My immediate reply was
> "DUH.... I don't know."
You didn't specify what cells you are working with - but if they are
lymphocytes they will nicely remove it themselves if you incubate them at 37
degrees C. in Ig-free medium for about an hour. The Fc bound IgG is
pinocytosed and degraded rapidly.
Another published technique is to briefly expose the cells to low pH. I cannot
quickly find the reference but if it is important to you I will dig it up.
Treating the cells with a protease (we used to use pronase) will remove not
only the Fc Bound Ig but the receptor itself and a great many other surface
proteins. Culturing the cells will then allow them to regenerate their surface
receptors but not the Fc bound Ig.
Hope this is helpful!
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Kenneth A. Ault M.D.
Maine Medical Center Research Institute
125 John Roberts Road
South Portland ME 04106
Voice (207) 761-9090
Fax (207) 761-2130
E-Mail aultk.mmcri@office.mmc.org
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