 |
 |
 |
 |
We have tried with occasional success lowering the pH to around 3
with HCl added to balanced salt solution. The IgG should in principle
dissociate from a type II receptor (for aggregated IgGs) under these
conditions. I am at home currently and don't have access to my methods
books but I think I got this procedure from the Cold Spring Harbor
Antibody Manual.
Steve Kayes
On Fri, 7 Apr 1995 jphillip@mednet.med.miami.edu wrote:
> One of the PI's from my department raised the question of "how to
> remove endogenous IgG from fc receptors?" My immediate reply was
> "DUH.... I don't know." But knowing that at my disposal was a vast
> network of immunological wizards, I said I would post the question and
> be flooded by precise and knowledgeable information, protocols,
> references, and "how to" responses. If anyone knows would you please
> help. Thank you. Jim
>
>
>
>
|
|
|
|