Re: removal of IgG from fc receptors

* (@VM.CC.PURDUE.EDU:HADAM@DHVMHH1)
Mon, 10 Apr 95 21:38:08 MEZ

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Another way to strip cells very efficiently from IgG is hypotonic
treatment. Take care to keep everything ice-cold and restore
isotonicity after 30 sec. Do not extend hypotonic conditions any
longer (this _is_ critical; use a stopwatch). Wash once with
ice-cold medium.
We used this procedure way back when Fc-receptors where still
quantitated using EA-rosettes.

Hope this helps-

Martin R. Hadam

Dr. Martin R. Hadam
Medizinische Hochschule Hannover
Kinderklinik
D-30623 HANNOVER
TEL (49) 511 532 9009 E-MAIL HADAM@DHVMHH1.BITNET
FAX (49) 511 532 9016 HADAM@VM.MHRZ.MH-HANNOVER.DE
Bundesrepublik Deutschland

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu