Aggregation by flow
Eric Martz (emartz@microbio.umass.edu)
Tue, 28 Mar 1995 11:14:32 -0500 (EST)
We've had good results measuring cell aggregation as 'single cell loss'
by flow. The main caveat is that this method is very sensitive to small
amounts of aggregation, since even pairs of cells count as aggregates. To
ensure that we get a value proportional to single cells/ml, we add a fixed
density of 2 micrometer beads to each sample just before applying
controlled vortex shear and acquiring. The latest version of MFI (version
3.4I) calculates gate ratios, e.g. single cells per bead, which makes this
type of analysis more convenient. The latest MFI Tutorial now includes a
new chapter 4 illustrating this method with sample data files.
MFI is available by anonymous ftp from flowcyt.bio.umass.edu in
/pub/flowcyt/mfi. It can also be obtained through our Free Flow Cytometry
Software Catalog, http://www.bio.umass.edu (select Flow Cytometry Facility,
then the Catalog).
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
