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What is the precision of the staining and how many replicates do you prepare
per experiment?
Flow cytometry *IS* limited in sensitivity (to about 5,000 FITC per particle)
and may not be the appropriate assay system. Positive/negative for fluorescence
does *NOT* mean that the antigen is or is not there!
Do you have this:
Flow Cytometry Titration of Retroviral Expression Vectors: Comparison of Methods
for Analysis of Immunofluorescence Histograms Derived from Cells Expressing Low
Antigen Levels, Sladek and Jacobberger, Cytometry, 14:23-31 ,1993)?
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* Dennis J. Young Voice : (619) 543-3928 *
* Flow Cytometry Core Facility FAX : (619) 543-7487 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
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