membrane and nuclear protein

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Does anyone know if double stain of membrane protein and nuclear protien is
possible?

I am trying simultanous double stain of membrane protein and DNA polymerase
alpha. Antibody for membrane protein is conjugated with biotin, so it is
detectable by avidin-Texas Red. Antibody for DNA polymerase alpha is
conjugated with FITC. So I think double stain is possible in theory, but
the biggest problem is fixtaion. I cannot find such articles about it.

Any information is welcome. Thank you.
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HARUTAKA KATANO, D.D.S.
Department of Pathology
Institute of Medical Science,
University of Tokyo.
4-6-1 Shirokane-dai,
Minato-ku, Tokyo 108.
Fax; +81-3-5449-5418
Email:katano@ims.u-tokyo.ac.jp
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