 |
 |
 |
 |
I would like to hear from other Time of Flight-users what their experience
is, concerning the size-range you can measure in 1 run. On our Flow Cytometer
the TOF gives only lineair output of data, while our samples have hughe
ranges ( from bacteria <1 mu to filamentous cyanobacteria >400 mu ).
Depending on the setting we go from .5 to 20 mu, or from 5 to 40 etc. An
logarithmic output , like with other parameters 4 decade, would make it
easier. You can of course run a sample more times with different settings,
but then the overview is lost.
Has anyone such a logarithmic possibility ( or has heard about it), or do
you think it is possible to modify our FCM (Coulter Elite) in this way?
What are the other possibilties (if any) and limits to measure size?
Thanking in advance,
Hans
Hans Hoogveld
Netherlands Institute of Ecology, Centre for Limnology (NIOO-CL)
Flowcytometry
Rijksstraatweg 6 voice : +31 2943 3599
3631 AC NIEUWERSLUIS fax : +31 2943 2224
The Netherlands internet : hoogveld@cl.nioo.nl
|
|
|
|