Alternative to BrdU labelling in vivo ?

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I am trying to determine the cycling population of CD8 T cells in vivo. As
you know, BrdU labelling involves the fixation of cells for staining, and I
am wondering if anyone out there knows any alternative labelling methods
which do not require the fixation of cells. Thanks in advance !!

Aki Hoji (wondering when spring comes here)
University of Wisconsin, Madison.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu