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Use chlorine dioxide solutions (100-1000 mg/l). ClO2, a yellow gas.
freely soluble in water, also soluble in non polar solvents, diffuses
into lipid membranes, and is a far more more powerful oxidant and
microbicide than hypochlorite (CHLOROX BLEACH). It is a peroxy
radical transfer agent of one of the highest redox potentials known.
It bleaches rapidly most dyes and fluorochromes, however without
covalently chlorinating unsaturated groups, as hypochlorite
unfortunately does. It is a superior penetrating agent for bleaching
and disinfection of paper, plastics,etc, as it is non-ionic (Note
ClO2 is a mixed covalent anhydride of chlorous and chloric acids, and
as such it is a non polar molecule). Chemically it is mild, its pH is
about 4-5 in distilled water. It must be stressed that it does not
attack (chlorinate and yellow) plastic parts as chlorox bleach
(pH>10) would. It is very easy and cheap and safe to prepare: Place
50 ml of 3% NaClO2 (sodium chlorite) in a bubble purge trap (several
kinds available from Fisher Co.), add 10 ml of 20% H2SO4, the
solution turns bright yellow. Slowly bubble air thru this solution
and trap the purged yellow off gases containing gaseous ClO2 into 100
ml deionized water. A visibly yellow solution is obtained after 10-15
min, this contains about 100-1000 mg/l of ClO2 (depends on the
duration of purge). This solution is stable indefinitely, provided it
is shielded from light. Avoid inhaling ClO2, do not generate
excessively high concentrations, do not exceed the above
concentrations. Try this powerful and ultra-fast disinfectant where
CHLOROX fails, it may also effectively fade your residual acridine
orange fluorescence sticking to tubing and flow cells, at the same
time offering superior broad spectrum bactericidal and virucidal
properties. Disadvantages: Not available commercially, you've got to
prepare it yourself.
PRECAUTIONS: DO NOT INHALE, DO NOT SNIFF THE SOLUTION, its pungent
and can temporarily ruin your sense of smell.
IN VERY HIGH CONCENTRATIONS (several gm/l telltale: the solution is
intense dark red/brown/yellow) IT IS EXPLOSIVE.
Otherwise its quite safe, skin contact is innocent, it has been used
to disinfect skin lesions.TRY IT, YOU MAY LIKE IT.
>>>>>>>>>>>>>>>
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I am currently working with the dye thiazole orange, and so far the
research project has been limited to a FACScan. However, we'd like
to move on to the next step, which would involve sorting on a B-D
FACS440. I have found a few articles in the literature where
individuals have used this dye on a FACSTAR
PLUS. Has anyone ever used this dye on a sorter? I thought it was
in the same chemical family as acridine orange, and I know that
acridine orange gets embedded in the tygon tubing and results in
tubing replacement. The FACS440 has even more fluidic tubing than
the FACSTAR PLUS. I was planning on just running 10% bleach through
the fluidics after the sort. Is this adequate for removing all
residual dye?
The working concentration we are using is 1 ug/ml. Thanks so much
for any comments on this topic.
Marilyn Dietrich
Louisiana State University dietrich@vt8200.dnet.lsu.edu
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