Analysis for dim fluorescen

Susan Grigsby (susan_grigsby@iucc.iupui.edu)
Tue, 28 Feb 1995 17:48:00 -0500

2/28/95 4:40 PM
IUCC Re: Analysis for dim fluorescence

Hello!
I have an investigator who is interested in calculating the % positive of a
very dim marker as compared to an isotype control where the control and test
may greatly overlap. A lab in Moscow used an analysis technique where the
test histagram was overlayed on the control histagram and the area under the
curves was compared to arrive at a % positive for the test. I understand that
the control and test were run separately and then compared from the list mode
stored data. He does not recall how this was done. I have a FACStar Plus
and a FACscan. Any advice or references would be greatly appreciated. Thanks
for your help.
Susan

Indiana University Cancer Center


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