RE: NK marker in A/J mice
kukuruga%kasle1.dnet@rocdec.roc.wayne.edu
Thu, 23 Feb 95 11:34:46 -0500
In responce to the NK query...we have had goo success with the WAKO anti-
AsialoGM1, on most strains. It does target other cell types, though,
so you have to combine with CD3 as a two-color, or MAC1 for monocytes.
This is best anyway, since these populatiuons are fairly dynamic in most
cases. The NK population will be the brightest expressing CD3+ or MAC1-
population. We have also tried an NK1.1 antibody (personal source), but
had little success, and have heard others with similar results.
Mark KuKuruga
kukuruga@oncgate.roc.wayne.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
