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To try to aid our decision making, we hope we can persuade some of you to
complete the attached survey. Our intention is that for most points, or
for most of those you wish to respond to, you score your satisfaction with
the feature, on a scale from 0 to 10.
0 Not applicable
1 Totally unsatisfactory
2...4
5 Satisfactory
6...9
10 Brilliant
A few questions require descriptive replies.
We will, of course, guarantee the confidentiality of any specific opinions
offered, but equally would be glad to provide summary results to any
respondents.
I have never tried to send such a long message before. Will it be more
than the bulletin board can chew?? Fingers crossed....
with thanks
Andrew Collins
School of Microbiology and Immunology
University of New South Wales
Sydney 2052 AUSTRALIA
=======================================
What sorter are you using? ..............................
PLEASE INDICATE YOUR LEVEL OF SATISFACTION WITH THE FOLLOWING:
ITEM
OPTICAL BENCH
baseplate features ...
laser mounts ...
steering mirrors ...
beams enclosures ...
stream visualisation ...
FLUIDICS
fluidics monitors/warning ...
sample cooling ...
sample mixing ...
sheath filtering ...
autoclaving ...
sample line dead volume ...
containment of sheath collection area ...
sample line replacement (ease/time) ...
sort collection cooling ...
FLOW CELL
range available ...
SIGNAL DETECTION
filter sets and dichroics ...
bead standards ...
alignment display features ...
signal display ...
signal count display ...
coincidence count ...
ALIGNMENT
major adjustments (#, type) single beam ...
ditto, two beams displaced ...
major alignment features and simplicity ...
ditto, two beams displaced ...
routine daily adjustments (#, type) ...
time for daily alignments ...
fluorescence calibration ...
time for realignment after blockage clearance ...
second beam add-on time (displaced) ...
SIGNAL PROCESSING AND ACQUISITION
time as parameter ...
multiple processing of one signal ...
intra-beam compensation (tabulate) ...
inter-beam compensation (dual laser) ...
SORTING (single beam)
max. purity achieved on your machine ...
max. sort rate at this purity ...
max. signal rate at this sort rate ...
normal recovery ...
Do you sort in quartz or air? ...
SORTING (dual beam displaced)
max. purity achieved on your machine ...
max. sort rate at this purity ...
max. signal rate at this sort rate ...
normal recovery ...
Do you sort in quartz or air? ...
SORTING - GENERAL FEATURES
esp/sem ...
macrosort ...
autosort ...
counts and displays ...
autoclone ...
computer analysis in background ...
COMPUTER ACQUISITION
monitoring functions operating, analysis ...
monitoring sorting ...
listmode capabilities ...
COMPUTER CONTROLS
pots, gains, PMT settings, etc ...
sort populations ...
amorphous sort regions ...
software set-up features ...
display of sort performance ...
monitoring of whole sort ...
COMPUTER ANALYSIS AND SOFTWARE
max number usable parameters ...
max # histograms on screen ...
max # bivariates ...
pseudo-3D and #s ...
colour-gating ...
dotplots, contours ...
live vs list as source: differences ...
isometrics ...
phenotyping ...
DNA ...
kinetics ...
amorphous gating ...
derived parameters ...
USER INTERFACE FEATURES
ease of user input ...
hardcopy publication quality ...
macro/protocol storage and recovery ...
transfer and recall of machine settings ...
FCS file formats offered ...
interface to other packages ...
ACCESSORIES
offline license for analysis software ...
networking ...
warranty periods ...
software upgrade policy ...
Other Comments or Features.......
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