Apoptosis

Sailer Brian (D9AT@SDSUMUS.SDSTATE.EDU)
Thu, 12 Jan 95 07:00:46 CST

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From: shenker@biochem.dental.upenn.edu (Bruce Shenker)
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Subject: Apoptosis
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Date: Tue, 10 Jan 1995 17:18:04 -0500 (EST)
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--------------------------- Original Message ---------------------------
--------------------------- Original Message ---------------------------
I am interested in utlizing the FACS to identify cells undergoing
apoptosis. We have tried the Oncor Apotag Kit to label fragmented DNA
and have had mixed. Does anyone have experience with this system or any
other kit/system to label fragmented DNA for analysis by flow.?

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    _/_/_/_/   _/_/_/_/   _/    _/   _/    _/Bruce J. Shenker Ph.D.
   _/    _/   _/         _/_/  _/   _/_/  _/ shenker@biochem.dental.upenn.edu
  _/_/_/_/   _/_/_/     _/ _/ _/   _/ _/ _/  (215)898 5959;FAX 573 2050
 _/         _/         _/  _/_/   _/  _/_/   School Of Dental Medicine
_/         _/_/_/_/   _/    _/   _/    _/    THE UNIVERSITY OF PENNSYLVANIA

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This kit has worked for others at our facility using our Facsort.
Other methods would be to use AO staining, or the TdTA or nick
translation assays (see review by Darzynkiewicz et al. Features of
apoptotic cells measured by flow cytometry. Cytometry 13:795-
808, 1992).



======================================================================
Brian Sailer
d9at@sdsumus.sdstate.edu
South Dakota State University
Broookings, SD 57007

In Alaska, if you ain't the lead dog the view never changes!
=======================================================================

======================================================================
Brian Sailer
d9at@sdsumus.sdstate.edu
South Dakota State University
Broookings, SD 57007

In Alaska, if you ain't the lead dog the view never changes!
=======================================================================

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu