Re: Apoptosis

Dave Coder (dave@nucleus.immunol.washington.edu)
Wed, 11 Jan 95 08:41:36 -0800

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I'm unfamiliar with the Oncor kit, but the method using Hoechst 333442 to
label apoptotic cells and 7-AAD to distinguish live from dead has worked quit
well in our hands. This requires, of course, a dual laser system (uv and
488nm excitation). We have used these probes in conjunction with two surface
markers (FITC and PE labels) to identify differences in apoptosis among
phenotypes. The basic method is described by Schmid et al. Cytometry. 1994
15(1):12-20.

Dave Coder

Begin forwarded message:

From: shenker@biochem.dental.upenn.edu (Bruce Shenker)
Subject: Apoptosis
To: cytometry@flowcyt.cyto.purdue.edu (cytometry)
Date: Tue, 10 Jan 1995 17:18:04 -0500 (EST)
X-Mailer: ELM [version 2.4 PL23-upenn2.9]

I am interested in utlizing the FACS to identify cells undergoing
apoptosis. We have tried the Oncor Apotag Kit to label fragmented DNA
and have had mixed. Does anyone have experience with this system or any
other kit/system to label fragmented DNA for analysis by flow.?

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_/_/_/_/ _/_/_/_/ _/ _/ _/ _/Bruce J. Shenker Ph.D. _/ _/ _/ _/_/ _/ _/_/ _/ shenker@biochem.dental.upenn.edu _/_/_/_/ _/_/_/ _/ _/ _/ _/ _/ _/ (215)898 5959;FAX 573 2050 _/ _/ _/ _/_/ _/ _/_/ School Of Dental Medicine _/ _/_/_/_/ _/ _/ _/ _/ THE UNIVERSITY OF PENNSYLVANIA

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