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I have not located a commercially available mouse anti human IL-8R
antibody either monoclonal or polyclonal, but there is a fluorokine
receptor reagent available from R&D Syatems. These fluorokines are designed
for analyzing cytokine receptors on 6the cell surface quantitatively.
They use recombinant cytokines as receptor ligands and come in either
biotin or phycoerythrin conjugates. You can actually measure more than
one cytokine on the cell surface this way.
The protocol is simple. Incubate cells with the fluorokine. Wash to
remove unbound cytokine. If biotin add AV-fluorochrome incubate and
wash. Analyze on FACS. The intensity of the stain should be
proportional to the density of receptors.
Human Interleukin-8 fluorokine biotin is catalog #F8000 and comes with
Avidin-Fluorocein.
Human Interleukin-8 fluorokine phycoerythrin is catalog F8050P.
They both come with a wash buffer concentrate.
Good luck!
Rochelle Diamond
Applications Specialist
Flow Cytometry/Cell Sorting Facility
Division of Biology
California Institute of Technology
diamond@cco.caltech.edu
On Fri, 23 Dec 1994, Richard Moldwin wrote:
> Hi,
> I'm new to this list, so I hope these are appropriate questions:
>
> Does anyone know where I can obtain an antibody to the human IL-8 Receptor
> for use in Flow cytometry?
>
> If I can't find an antibody, I have an alternate idea:
> 1. incubate cells with IL-8
> 2. Incubate cells with polyclonal Rabbit anti-IL-8 antibody
> 3. Incubate cells with with FITC-conjugated Goat anti-Rabbit antibody
> --Does anyone have any experience with this type of approach to label any
> receptor molecule?
>
> Thanks in advance.
> --Rich
>
>
>
> Richard Moldwin, M.D., Ph.D.
> Assistant Professor of Pediatric Hematology/Oncology
> University of Chicago Hospitals
> 5841 S. Maryland Ave., M/C 4060
> Chicago, IL 60637
>
> E-mail: rmoldwin@midway.uchicago.edu
> Phone 312-702-6808
> Fax 312-702-9881
>
>
>
>
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