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I have been told that there are two types of anti-CD4 mAbs: those that bind
to an epitope proximal to the T-cell surface and those that bind to a more
distal epitope. As I am looking for an antibody that will bind to a site
that is resistant to enzyme digestion, I would like to try both classes of
mAb. Does anyone on the flow network have any information about these two
types of anti-CD4 Abs -- and how I can find out which commercial antibodies
fall into which category? Thanks.
Alice,
I haven't played with this for a few years, but the best paper
on the subject I can remember which should help you is as follows:
FUNCTIONAL EPITOPE ANALYSIS OF THE HUMAN CD4 MOLECULE - THE MHC
CLASS-II-DEPENDENT ACTIVATION OF RESTING T-CELLS IS INHIBITED BY
MONOCLONAL-ANTIBODIES TO CD4 REGARDLESS WHETHER OR NOT THEY RECOGNIZE
EPITOPES INVOLVED IN THE BINDING OF MHC CLASS-II OR HIV GP120
MERKENSCHLAGER_M, BUCK_D, BEVERLEY_PCL, SATTENTAU_QJ
JOURNAL OF IMMUNOLOGY 1990 Vol.145 No.9 pp.2839-2845
Have fun,
Mike Salmon
............................
Mike Salmon
Dept of Rheumatology
The University of Birmingham
Birmingham B15 2TT
United Kingdom
Tel 44 (0)21 414 6780
Fax 44 (0)21 414 6794
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