Sorting biohazards
Dean,Hewish (deanh@tigger.mel.dbe.CSIRO.au)
Thu, 17 Nov 1994 09:39:59 +1000
We sort recombinant cell lines all the time- it was the reason our
cytometer was bought in the first place. Although none of the expressed
proteins have been potentially hazardous to date, the cells are subject to
containments because of the regulatory guidelines and we do the usual
sterilising of the sort area before and after.
The Elite has an extraction fan that draws air through the sort chamber and
exhausts via a biohazard filter- not a perfect system but it is better than
nothing. Strangely, Coulter have placed the switch for the fan on the back
of the machine out of sight-and out of mind. I have rigged an LED across
the output terminals of the switch and have mounted it inside the sort
chamber so that there is an indication whether the fan is on. The fan
supply is 24V, so you need a 1K ohm resistor in series with the LED. It is
an easy modification and anyone can do it in a few minutes.
Dean R. Hewish, Cell Biologist & Flow Cytometrist.
CSIRO Biomolecular Engineering, 343 Royal Parade, Parkville, 3052,
Victoria Australia.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
